The TSA kits are designed for use in slide-based applications.  For ELISA applications, the PERKINELMER ELAST™ kit (ELISA Amplification SysTem) provides reagents optimized for use in the amplification of solution phase signal in microplate-based assays.  The ELAST reagents are easily inserted into standard chromogenic ELISA protocols, resulting in an 8 to 32-fold improvement of sensitivity.  ELAST uses immobilized HRP to catalyze the deposition of the biotin-labeled amplification reagent onto the solid phase, which has been previously blocked with protein. The deposited biotin labels can then be subsequently detected using Streptavidin-enzyme conjugate, followed by the appropriate solution phase chromogen such as OPD or TMB.  The kit (Cat.#NEP116) provides reagents sufficient for amplification of signal in ten 96-well microplates. Kit compoPerkinElmerts include the biotin-labeled tyramide amplification reagent, 2X amplification diluent, and streptavidin-HRP concentrate.